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NGAL Antibody (ABS 038-15-02) in ICC/IF
Immunofluorescence analysis of NGAL was performed using 70% confluent log phase HT-29 treated with Thapsigargin (500 nm, 48hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NGAL Monoclonal Antibody (15) (Product # ABS 038-15-02) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Al... View More
产品信息
ABS 038-15-02
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
15
抗原
Recombinant human NGAL adsorbed onto aluminum hydroxide gel.
偶联物
形式
Liquid
浓度
1.02 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4
内含物
15mM sodium azide
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536216
产品详细信息
Specificity: ABS 038-15-02 does not cross-react with NGAL from other mammalian species tested.
Reactivity: ABS 038-15-02 binds human NGAL in solution and coated onto a solid phase. ABS 038-15-02 can be used as a capture antibody in a sandwich ELISA using biotinylated detection antibodies HYB 211-02-02, ABS 038-04-02, ABS 038-14-02, ABS 038-23-02 and ABS 038-26-02. Using biotinylated ABS 038-15-02 as a detection antibody, the assay reacts equally with NGAL monomer and homodimer on a mass basis.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
靶标信息
NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HGNC:6526; Lipocalin-2; Lipocalin2; migration-stimulating factor inhibitor; Neutrophil gelatinase-associated lipocalin; NGAL; Oncogene 24p3; OTTHUMP00000022240; p25; RP23-161B9.11; Siderocalin; siderocalin LCN2; uterocalin
基因别名: 24p3; HNL; LCN2; MSFI; NGAL; p25
UniProt ID: (Human) P80188
Entrez Gene ID: (Human) 3934
